MDFIC antibody (Middle Region)

Catalog No. ABIN928479

Product Details anti-MDFIC Antibody

Target: MDFIC (MyoD Family Inhibitor Domain Containing (MDFIC))

Binding Specificity: Middle Region

Reactivity: Human, Rabbit, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDFIC antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Rabbit, Pig (Porcine), Cow (Bovine)

Purification: Purified

Immunogen: MDFIC antibody was raised in rabbit using the middle region of MDFIC as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

MDFIC Blocking Peptide, catalog no. 33R-7305, is also available for use as a blocking control in assays to test for specificity of this MDFIC antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for MDFIC

Target: MDFIC (MyoD Family Inhibitor Domain Containing (MDFIC))

Alternative Name: MDFIC (MDFIC Products)

Synonyms: HIC antibody, Kdt1 antibody, Mdfid antibody, MyoD family inhibitor domain containing antibody, MyoD family inhibitor domain containing S homeolog antibody, MDFIC antibody, Mdfic antibody, mdfic.S antibody

Background: MDFIC is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization, p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Synonyms: Polyclonal MDFIC antibody, Anti-MDFIC antibody, MyoD family inhibitor domain containing antibody, HIC antibody.