REST antibody (Middle Region)

Catalog No. ABIN927729

Product Details anti-REST Antibody

Target: REST (RE1-Silencing Transcription Factor (REST))

Binding Specificity: Middle Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine)

Purification: Purified

Immunogen: Rest antibody was raised in rabbit using the middle region of Rest as the immunogen

Application Details

Application Notes: Optimal conditions should be determined by the investigator.

Comment:

Rest Blocking Peptide, catalog no. 33R-1402, is also available for use as a blocking control in assays to test for specificity of this Rest antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: Rest (REST Products)

Synonyms: NRSF antibody, XBR antibody, 2610008J04Rik antibody, AA407358 antibody, xbr antibody, nrsf antibody, rest antibody, resta antibody, REST antibody, RE1 silencing transcription factor antibody, RE1-silencing transcription factor antibody, RE1-silencing transcription factor S homeolog antibody, REST antibody, Rest antibody, rest.S antibody, rest antibody, LOC615792 antibody

Background: Rest is a transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Rest restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Rest mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Synonyms: Polyclonal Rest antibody, Anti-Rest antibody, RE1-silencing transcription factor antibody, 2610008J04Rik antibody, AA407358 antibody, MGC150099 antibody, NRSF antibody.

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity