SUPT16H antibody (N-Term)

Catalog No. ABIN925767

Product Details anti-SUPT16H Antibody

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Dog (Canine), Mouse (Murine), Rat (Rattus), Zebrafish (Brachydanio rerio), Frog, Cow (Bovine)

Immunogen: SUPT16 H antibody was raised in rabbit using the N terminal of SUPT16 as the immunogen

Application Details

Application Notes: WB: 1.25 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

SUPT16H Blocking Peptide, catalog no. 33R-1511, is also available for use as a blocking control in assays to test for specificity of this SUPT16H antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 100 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: SUPT16H (SUPT16H Products)

Background: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is SUPT16H. Synonyms: Polyclonal SUPT16H antibody, Anti-SUPT16H antibody, suppressor of Ty 16 homolog, S. cerevisiae antibody, CDC68 antibody, FACT antibody, FACTP140 antibody, FLJ10857 antibody, FLJ14010 antibody, FLJ34357 antibody, SPT16/CDC68 antibody.

Pathways: Chromatin Binding