DYKDDDDK Tag antibody (FITC)
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- Target See all DYKDDDDK Tag products
- DYKDDDDK Tag
- Reactivity
- Please inquire
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DYKDDDDK Tag antibody is conjugated to FITC
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Application
- Western Blotting (WB), ELISA
- Sequence
- DYKDDDDK
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- Application Notes
- ELISA: Use at a 1:5,000 - 1:20,000 dilution. Western blot: Use at a 1:1,000 - 1:5,000 dilution.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.1 mL at 1.0 mg/mL FITC-conjugated polyclonal antibody in PBS, pH 7.0 with protein stabilizer and 0.1% sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- DYKDDDDK Tag
- Alternative Name
- Flag Tag (DYKDDDDK Tag Products)
- Target Type
- Tag
- Background
- Rabbits were immunized with the DYKDDDDK (DYKDDDDK) peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, serum was collected and the antibodies were affinity-purified using the peptide immobilized on a solid phase. The affinity-purified antibodies were then conjugated with Fluorescein Isothiocyanate (FITC). Prior to conjugation, the antibodies were analyzed by ELISA and Western blot. Specification: The molar F/P ratio is 3.14.
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