Vimentin antibody

Catalog No. ABIN920739

Product Details anti-Vimentin Antibody

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells, and has a molecular weight of 57 kDa. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments. The antibody stains the 57 kDa vimentin band in immunoblots performed on a lysate of normal human lymphocytes. No detectable cross-reactivity with GFAP, Keratin, Neurofilament or Desmin.

Purification: Purified

Clone: V9

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: For use on frozen sections and formalin-fixed/paraffin- embedded tissues if pre-treated with trypsin. Paraffin sections: Use at an approximate dilution of 1:50 - 1:100 in PBS. Trypsin digestion may be required. Frozen sections: Use at an approximate dilution of 1:300 in PBS.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 mL of purified monoclonal antibody at approximately 30 µg/mL in PBS containing 20 mM sodium azide, and protein stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin (VIM Products)

Pathways: Caspase Cascade in Apoptosis