Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
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Vandekerckhove, Weber: "At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide." in: Journal of molecular biology, Vol. 126, Issue 4, pp. 783-802, (1979) (PubMed).