Streptavidin antibody (HRP)

Catalog No. ABIN916779

Product Details anti-Streptavidin Antibody (HRP)

Target: Streptavidin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Streptavidin antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Cross-Reactivity (Details): Streptavidin

Purification: Purified by Protein A.

Immunogen: Recombinant Streptavidin

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Streptavidin antibody (HRP) (ABIN916779)

Wang, Li, Zhang, Li, Yue, Zhang, Zhao, Wang, Duan, Li, Duan: "Caspase-1 inhibitor ameliorates experimental autoimmune myasthenia gravis by innate dendric cell IL-1-IL-17 pathway." in: Journal of neuroinflammation, Vol. 12, pp. 118, (2016) (PubMed).

Target Details for Streptavidin

Target: Streptavidin

Abstract: Streptavidin Products

Background:

Synonyms: SA protein, SA V1, SA V2, Streptavidin V1, Streptavidin V2, SAV1_STRVL.

Background: Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.