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Protein A antibody (AbBy Fluor® 555)

Reactivity: Staphylococcus aureus WB, IF (p), IF (cc) Host: Rabbit Polyclonal AbBy Fluor® 555
Catalog No. ABIN912267
  • Target See all Protein A Antibodies
    Protein A
    Reactivity
    • 49
    • 5
    • 1
    Staphylococcus aureus
    Host
    • 19
    • 15
    • 14
    • 5
    • 2
    Rabbit
    Clonality
    • 48
    • 2
    Polyclonal
    Conjugate
    • 11
    • 8
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Protein A antibody is conjugated to AbBy Fluor® 555
    Application
    • 35
    • 34
    • 12
    • 12
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
    Cross-Reactivity
    Bacteria
    Cross-Reactivity (Details)
    S. aureus
    Purification
    Purified by Protein A.
    Immunogen
    Recombinant protein A
    Isotype
    IgG
    Top Product
    Discover our top product Protein A Primary Antibody
  • Application Notes
    IF(IHC-P) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    Protein A
    Abstract
    Protein A Products
    Synonyms
    alpha protein antibody, repB antibody
    Background

    Synonyms: Immunoglobulin G binding protein A, Staphylococcal protein A, SPA, gG-binding protein A, Staphylococcus aureus.

    Background: Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.

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