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EGFRviii antibody (AA 1-50) (AbBy Fluor® 350)

EGFRv3 Reactivity: Human WB, FACS, IF (cc), IF (p) Host: Rabbit Polyclonal AbBy Fluor® 350
Catalog No. ABIN895022
  • Target See all EGFRviii (EGFRv3) products
    EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))
    Binding Specificity
    • 14
    • 3
    AA 1-50
    Reactivity
    Human
    Host
    • 17
    Rabbit
    Clonality
    • 17
    Polyclonal
    Conjugate
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This EGFRviii antibody is conjugated to AbBy Fluor® 350
    Application
    • 17
    • 12
    • 12
    • 12
    • 6
    • 3
    • 3
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Cross-Reactivity
    Chicken, Human, Mouse
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human EGFRvIII
    Isotype
    IgG
  • Application Notes
    IF(IHC-P) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))
    Abstract
    EGFRv3 Products
    Background

    Synonyms: EGFR vIII, EGFRvIII, Epidermal growth factor receptor variant type _, epidermal growth factor receptor isoform a variant, ERBB, HER1, ERBB1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR

    Background: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

    Gene ID
    1956
    UniProt
    Q59FL8
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