DBF4 antibody (AA 21-110) (AbBy Fluor® 488)

Catalog No. ABIN893547

Product Details anti-DBF4 Antibody (AbBy Fluor® 488)

Target: DBF4 (DBF4 Homolog (DBF4))

Binding Specificity: AA 21-110

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBF4 antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DBF4A

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: DBF4A (DBF4 Products)

Background:

Synonyms: Activator of S phase kinase, ASK, Chfon homolog A, DBF4, DBF4 type zinc finger containing protein 1, DBF4-type zinc finger-containing protein 1, DBF4A, DBF4A_HUMAN, Protein DBF4 homolog A, ZDBF1.

Background: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.Tissue specificity: Highly expressed in testis and thymus. Expressed also in most cancer cells lines.

Gene ID: 10926

Pathways: Mitotic G1-G1/S Phases, DNA Replication