HNE antibody (Biotin)

Catalog No. ABIN880510

Product Details anti-HNE Antibody (Biotin)

Target: HNE (4-Hydroxynonenal (HNE))

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNE antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Monkey, Mouse, Rat

Cross-Reactivity (Details): 4-Hydroxynonenal

Purification: Purified by Protein A.

Immunogen: 4 Hydroxynonenal conjugated to BSA

Isotype: IgG

Application Details

Application Notes: IHC-P 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for HNE

Target: HNE (4-Hydroxynonenal (HNE))

Alternative Name: 4 Hydroxynonenal (HNE Products)

Target Type: Chemical

Background:

Synonyms: 4-Hydroxy-2-Nonenal, 4Hydroxynonenal, 4-Hydroxynonenal, 4 HNE, 4HNE, 4-HNE, E-4-Hydroxynonenal-dimethylacetal, 4-HNE-DMA.

Background: Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress 4-HNE has been hypothesized to play a key role in cell signal transduction, in a variety of pathways from cell cycle events to cellular adhesion.