Streptavidin antibody

Catalog No. ABIN872946

Product Details anti-Streptavidin Antibody

Target: Streptavidin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Streptavidin antibody is un-conjugated

Application: ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Cross-Reactivity (Details): Streptavidin

Purification: Purified by Protein A.

Immunogen: Recombinant Streptavidin

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Streptavidin antibody (ABIN872946)

Fang, Pan, Lin, Zhang, Rauvala, Schachner, Shen: "HMGB1 contributes to regeneration after spinal cord injury in adult zebrafish." in: Molecular neurobiology, Vol. 49, Issue 1, pp. 472-83, (2014) (PubMed).

Pan, Lin, Ma, Shen, Schachner: "Major vault protein promotes locomotor recovery and regeneration after spinal cord injury in adult zebrafish." in: The European journal of neuroscience, Vol. 37, Issue 2, pp. 203-11, (2013) (PubMed).

Target Details for Streptavidin

Target: Streptavidin

Abstract: Streptavidin Products

Target Type: Tag

Background:

Synonyms: SA protein, SA V1, SA V2, Streptavidin V1, Streptavidin V2, SAV1_STRVL.

Background: Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.