SKA3 antibody (AA 201-300)

Catalog No. ABIN872869

Product Details anti-SKA3 Antibody

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Binding Specificity: AA 201-300

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKA3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SKA3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SKA3 antibody (ABIN872869)

Hou, Wang, Huang, Sun, Zhao, Shi, Li, Wang, He, Tam, Wu: "SKA3 Promotes tumor growth by regulating CDK2/P53 phosphorylation in hepatocellular carcinoma." in: Cell death & disease, Vol. 10, Issue 12, pp. 929, (2020) (PubMed).

Target Details for SKA3

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Alternative Name: SKA3 (SKA3 Products)

Synonyms: C13orf3 antibody, RAMA1 antibody, RGD1307201 antibody, C12H13ORF3 antibody, fc23d11 antibody, si:rp71-68n21.13 antibody, wu:fc23d11 antibody, rama1 antibody, F630043A04Rik antibody, spindle and kinetochore associated complex subunit 3 antibody, spindle and kinetochore associated complex subunit 3 L homeolog antibody, SKA3 antibody, Ska3 antibody, ska3 antibody, ska3.L antibody

Background:

Synonyms: C13orf3, RAMA1, SKA3, SKA3_HUMAN, Spindle and kinetochore associated complex subunit 3, Spindle and kinetochore-associated protein 3.

Background: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.