Biglycan antibody (AA 101-200)

Catalog No. ABIN872817

Product Details anti-Biglycan Antibody

Target: Biglycan (BGN)

Binding Specificity: AA 101-200

Reactivity: Human, Rat, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biglycan antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Biglycan

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Biglycan antibody (ABIN872817)

Han, Wang, Jiang, Ma, Tian, Zang, Ma: "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." in: Scientific reports, Vol. 6, pp. 35102, (2016) (PubMed).

Huang, Zou, Shi, Zhang, Pen, Zhang, Gao, Wang: "The effect of electroacupuncture on the extracellular matrix synthesis and degradation in a rabbit model of disc degeneration." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 731395, (2014) (PubMed).

Target Details for Biglycan

Target: Biglycan (BGN)

Alternative Name: Biglycan (BGN Products)

Background:

Synonyms: PGI, DSPG1, PG-S1, SLRR1A, Biglycan, Bone/cartilage proteoglycan I, BGN

Background: The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex.

Gene ID: 633

UniProt: P21810

Pathways: Glycosaminoglycan Metabolic Process