DNASE2 antibody (AA 25-90)

Catalog No. ABIN872658

Product Details anti-DNASE2 Antibody

Target: DNASE2 (Deoxyribonuclease II, Lysosomal (DNASE2))

Binding Specificity: AA 25-90

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNASE2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNase II alpha

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-DNASE2 antibody (ABIN872658)

Takahashi, Loo, Okada, Kamachi, Watanabe, Wakita, Watanabe, Kawamoto, Miyata, Barber, Ohtani, Hara: "Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells." in: Nature communications, Vol. 9, Issue 1, pp. 1249, (2018) (PubMed).

Target Details for DNASE2

Target: DNASE2 (Deoxyribonuclease II, Lysosomal (DNASE2))

Alternative Name: DNase 2 alpha (DNASE2 Products)

Background:

Synonyms: DNL, DNL2, DNASE2A, Deoxyribonuclease-2-alpha, Acid DNase, Deoxyribonuclease II alpha, DNase II alpha, Lysosomal DNase II, R31240_2, DNASE2

Background: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.

Gene ID: 1777

UniProt: O00115