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SPARC antibody (AA 52-56)
SPARC
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-SPARC Antibody
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Target
See all SPARC Antibodies
SPARC
(Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
Binding Specificity
All epitopes for SPARC antibodies
AA 52-56
Reactivity
All reactivities for SPARC antibodies
Human
Host
All hosts for SPARC antibodies
Rabbit
Clonality
All clonalities for SPARC antibodies
Polyclonal
Conjugate
All conjugates for SPARC antibodies
This SPARC antibody is un-conjugated
Application
All applications for SPARC antibodies
Western Blotting (WB)
Specificity
The antibody detects endogenous level of total SPARC protein.
Purification
Affinity purified
Immunogen
Peptide sequence around AA 52-56 (V-G-E-F-D) derived from Human SPARC. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
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Alternatives
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Application Details
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Application Notes
Western blotting: 1:500-1:1000
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target Details for SPARC
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Target
SPARC
(Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
Alternative Name
SPARC (SPARC Products )
Synonyms
ON antibody, AA517111 antibody, BM-40 antibody, hm:zeh0062 antibody, SPARC antibody, sparc antibody, secreted protein acidic and cysteine rich antibody, secreted acidic cysteine rich glycoprotein antibody, secreted protein, acidic, cysteine-rich (osteonectin) antibody, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog antibody, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog antibody, SPARC antibody, Sparc antibody, sparc antibody, sparc.L antibody, sparc.S antibody
Background
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites, an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Molecular Weight
42 kDa
Gene ID
6678
NCBI Accession
NP_003109
UniProt
P09486
Pathways
Autophagy
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