PRODH antibody (AA 112-134)

Catalog No. ABIN870655

Product Details anti-PRODH Antibody

Target: PRODH (Proline Dehydrogenase (Oxidase) 1 (PRODH))

Binding Specificity: AA 112-134

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRODH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: PRODH (aa112-134)

Sequence: EDQESIQPLL RHYR

Specificity: This antibody is expected to recognize both reported isoforms (NP_057419.4, NP_001182155.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-EDQESIQPLLRHYR, from the internal region of the protein sequence according to NP_057419.4, NP_001182155.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx75+55 kDa bands observed in Human Brain (Cerebellum and Substantia nigra) lysates (calculated MW of 68.0 kDakDa according to NP_057419.4 and of 56.2 kDa according to NP_001182155.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PRODH antibody (ABIN870655)

Celińska-Janowicz, Zaręba, Lazarek, Teul, Tomczyk, Pałka, Miltyk: "Constituents of Propolis: Chrysin, Caffeic Acid, p-Coumaric Acid, and Ferulic Acid Induce PRODH/POX-Dependent Apoptosis in Human Tongue Squamous Cell Carcinoma Cell (CAL-27)." in: Frontiers in pharmacology, Vol. 9, pp. 336, (2018) (PubMed).

Target Details for PRODH

Target: PRODH (Proline Dehydrogenase (Oxidase) 1 (PRODH))

Alternative Name: PRODH (PRODH Products)

Background: PRODH, proline dehydrogenase (oxidase) 1, FLJ33744, HSPOX2, MGC148078, MGC148079, PIG6, POX, PRODH1, PRODH2, SCZD4, TP53I6, OTTHUMP00000196496, OTTHUMP00000196497, p53-induced gene 6 protein, proline dehydrogenase, mitochondrial, proline oxidase 2, prolin

Molecular Weight: 68.0kDa

Gene ID: 5625

NCBI Accession: NP_057419, NP_001182155

Pathways: Response to Water Deprivation, Monocarboxylic Acid Catabolic Process