Myosin IB antibody (Internal Region)

Catalog No. ABIN870635

Product Details anti-Myosin IB Antibody

Target: Myosin IB (MYO1B)

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Myosin IB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: MYO1B

Sequence: RTVEAKQEKV STT

Specificity: This antibody is expected to recognize both reported isoforms (NP_001123630.1, NP_036355.2). Reported variants represent identical protein: NP_001123630.1, NP_001155291.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RTVEAKQEKVSTT, from the internal region of the protein sequence according to NP_001123630.1, NP_036355.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in the neuronal bodies. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 120 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 125 kDa according to Human NP_036355.2 and 128 kDa according to Mouse NP_034993.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Myosin IB

Target: Myosin IB (MYO1B)

Alternative Name: MYO1B (MYO1B Products)

Synonyms: MYO1B antibody, DKEY-242N5.2 antibody, myo1b2 antibody, myo1bl1 antibody, myo1bl2 antibody, si:busm1-60j23.1 antibody, wu:fb39h10 antibody, wu:fc46c09 antibody, zgc:110446 antibody, zgc:113187 antibody, myr1 antibody, AA406997 antibody, myosin-1b antibody, Myr1 antibody, myosin IB antibody, myosin Ib antibody, MYO1B antibody, myo1b antibody, DICPUDRAFT_46917 antibody, Myo1b antibody

Background: MYO1B, myosin IB, myr1, MMI-alpha, MMIa, MYH-1c, MYO1B variant protein, OTTHUMP00000165861, myosin I alpha, myosin-I alpha, myosin-Ib

Molecular Weight: 125kDa

Gene ID: 4430, 17912, 117057

NCBI Accession: NP_001123630, NP_036355