AQP3 antibody (C-Term)
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- Target See all AQP3 Antibodies
- AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
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Binding Specificity
- C-Term
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This AQP3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Detects ~31.5 kDa. May detect larger glycosylated bands ~35-50 kDa.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Protein A Purified
- Immunogen
- Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
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- Application Notes
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- WB (1:2000)
- IHC (1:200)
- ICC/IF (1:400)
- optimal dilutions for assays should be determined by the user.
- Comment
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0.5 μg/ml of ABIN863208 was sufficient for detection of aquaporin 3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- by
- Centre de Recherche en Transplantation et Immunologie, UMR1064, Université de Nantes
- No.
- #102635
- Date
- 12/14/2018
- Antigen
- AQP3
- Lot Number
- 160308
- Method validated
- Immunofluorescence
- Positive Control
Membrane staining in compacted morula; membrane staining apical side, on early blastocysts
- Negative Control
Cells not expressing AQP3 mRNA within human embryos (inner cell mass)
No primary control
- Notes
Passed. ABIN863208 specifically labels the targeted antigen in embryos in IF. No signal was detected in sample negative control tissue and the secondary antibody only control.
- Primary Antibody
- ABIN863208
- Secondary Antibody
- donkey anti-rabbit antibody (Life Technologies, A21206)
- Full Protocol
- Fixation
- Fix embryos in one drop of 30µl of 4% paraformaldehyde for 5min at RT.
- Remove embryos and wash quickly three times with PBS containing 0.1% BSA (in three drops of 30µl).
- Staining:
- Permeabilize embryo in 300µl IF buffer for 60min at RT.
- Incubate embryo with the primary rabbit anti-AQP3 antibody (antibodies-online, ABIN863208, 160308) diluted 1:400 in buffer ON at 4°C.
- Rinse embryo in IF buffer quickly three times.
- Incubate embryo with secondary donkey anti-rabbit antibody (Life Technologies, A21206) diluted 1:1000 in buffer for 2h at RT.
- Rinse embryo in PBS-BSA.
- At this point, the embryos can either be kept in PBS-BSA ON at 4°C or used for imaging directly on ibido µ-Slide.
- Image acquisition on a microscope using appropriate filters, 20x oil-immersion objective.
- Experimental Notes
Labeling of human embryo with the aquaporin 3 antibody ABIN863208 shows the expected staining pattern membrane-associated outside of the blastocyst. The background staining in our conditions is a bit high and might be improved with more stringent washes or different fixation-permeabilization protocols.
Validation #102635 (Immunofluorescence)Validation ImagesImmunoflurescence analysis of human embryo using ABIN863208. AQP3 (right) with nuclear counterstaining (left) at indicated stages. In the top panel (morula), the staining is located at the membrane. In the bottom panel (early blastocyst), the staining is located at the membrane, in trophectoderm cells. Scale bar = 47µm.Full Methods -
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- -20°C
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- Target
- AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
- Alternative Name
- Aquaporin 3 (AQP3 Products)
- Synonyms
- aqp3 antibody, wu:fa95h06 antibody, wu:fi13e05 antibody, zgc:55525 antibody, Tb06.28P18.240 antibody, AQP-2 antibody, AQP-3 antibody, GIL antibody, SAQP3 antibody, aquaporin 3a antibody, aquaporin 3 (Gill blood group) antibody, aquaporin 3 antibody, aqp3a antibody, AQP3 antibody, Tb927.6.1520 antibody, MCYG_07750 antibody, Aqp3 antibody
- Background
- Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells (3). AQP3 gene expression is not regulated by vasopressin (4).
- Gene ID
- 65133
- NCBI Accession
- NP_113891
- UniProt
- P47862
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