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AQP3 antibody (C-Term)

AQP3 Reactivity: Rat WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN863208
  • Target See all AQP3 Antibodies
    AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
    Binding Specificity
    • 17
    • 15
    • 9
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 51
    • 38
    • 35
    • 4
    • 4
    • 1
    • 1
    • 1
    Rat
    Host
    • 63
    Rabbit
    Clonality
    • 63
    Polyclonal
    Conjugate
    • 22
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AQP3 antibody is un-conjugated
    Application
    • 49
    • 26
    • 20
    • 18
    • 15
    • 13
    • 13
    • 8
    • 5
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~31.5 kDa. May detect larger glycosylated bands ~35-50 kDa.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein A Purified
    Immunogen
    Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
  • Application Notes
    • WB (1:2000)
    • IHC (1:200)
    • ICC/IF (1:400)
    • optimal dilutions for assays should be determined by the user.
    Comment

    0.5 μg/ml of ABIN863208 was sufficient for detection of aquaporin 3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Validation #102635 (Immunofluorescence)
    'Independent Validation' Badge
    by
    Centre de Recherche en Transplantation et Immunologie, UMR1064, Université de Nantes
    No.
    #102635
    Date
    12/14/2018
    Antigen
    AQP3
    Lot Number
    160308
    Method validated
    Immunofluorescence
    Positive Control

    Membrane staining in compacted morula; membrane staining apical side, on early blastocysts

    Negative Control

    Cells not expressing AQP3 mRNA within human embryos (inner cell mass)

    No primary control

    Notes

    Passed. ABIN863208 specifically labels the targeted antigen in embryos in IF. No signal was detected in sample negative control tissue and the secondary antibody only control.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN863208
    Secondary Antibody
    donkey anti-rabbit antibody (Life Technologies, A21206)
    Full Protocol
    • Fixation
      • Fix embryos in one drop of 30µl of 4% paraformaldehyde for 5min at RT.
      • Remove embryos and wash quickly three times with PBS containing 0.1% BSA (in three drops of 30µl).
    • Staining:
      • Permeabilize embryo in 300µl IF buffer for 60min at RT.
      • Incubate embryo with the primary rabbit anti-AQP3 antibody (antibodies-online, ABIN863208, 160308) diluted 1:400 in buffer ON at 4°C.
      • Rinse embryo in IF buffer quickly three times.
      • Incubate embryo with secondary donkey anti-rabbit antibody (Life Technologies, A21206) diluted 1:1000 in buffer for 2h at RT.
      • Rinse embryo in PBS-BSA.
      • At this point, the embryos can either be kept in PBS-BSA ON at 4°C or used for imaging directly on ibido µ-Slide.
      • Image acquisition on a microscope using appropriate filters, 20x oil-immersion objective.
    Experimental Notes

    Labeling of human embryo with the aquaporin 3 antibody ABIN863208 shows the expected staining pattern membrane-associated outside of the blastocyst. The background staining in our conditions is a bit high and might be improved with more stringent washes or different fixation-permeabilization protocols.

  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Target
    AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
    Alternative Name
    Aquaporin 3 (AQP3 Products)
    Synonyms
    aqp3 antibody, wu:fa95h06 antibody, wu:fi13e05 antibody, zgc:55525 antibody, Tb06.28P18.240 antibody, AQP-2 antibody, AQP-3 antibody, GIL antibody, SAQP3 antibody, aquaporin 3a antibody, aquaporin 3 (Gill blood group) antibody, aquaporin 3 antibody, aqp3a antibody, AQP3 antibody, Tb927.6.1520 antibody, MCYG_07750 antibody, Aqp3 antibody
    Background
    Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells (3). AQP3 gene expression is not regulated by vasopressin (4).
    Gene ID
    65133
    NCBI Accession
    NP_113891
    UniProt
    P47862
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