SLC12A3 antibody (AA 74-95)

Catalog No. ABIN863201

The Rabbit Polyclonal anti-SLC12A3 antibody has been validated for WB, IHC, ICC, IEM and IF. It is suitable to detect SLC12A3 in samples from Rat. There are 10+ publications available.

Quick Overview for SLC12A3 antibody (AA 74-95) (ABIN863201)

Target: SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC12A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM), Immunofluorescence (IF)

Product Details anti-SLC12A3 Antibody

Binding Specificity: AA 74-95

Specificity: Detects ~160 kDa.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Affinity Purified

Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN863201 was sufficient for detection of NCC3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-SLC12A3 antibody (ABIN863201)

Fenton, Poulsen, de la Mora Chavez, Soleimani, Dominguez Rieg, Rieg: "Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis." in: Kidney international, Vol. 92, Issue 2, pp. 397-414, (2018) (PubMed).

Procino, Carmosino, Milano, Dal Monte, Schena, Mastrodonato, Gerbino, Bagnoli, Svelto: "β3 adrenergic receptor in the kidney may be a new player in sympathetic regulation of renal function." in: Kidney international, Vol. 90, Issue 3, pp. 555-67, (2017) (PubMed).

Patel-Chamberlin, Varasteh Kia, Xu, Barone, Zahedi, Soleimani: "The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150918, (2016) (PubMed).

Gois, Canale, Luchi, Volpini, Veras, Costa, Shimizu, Seguro: "Tenofovir during pregnancy in rats: a novel pathway for programmed hypertension in the offspring." in: The Journal of antimicrobial chemotherapy, Vol. 70, Issue 4, pp. 1094-105, (2015) (PubMed).

Wen, Yuan, Warner, Wang, Cornelius, Wang-France, Li, Boettger, Sansom: "Increased Epithelial Sodium Channel Activity Contributes to Hypertension Caused by Na+-HCO3- Cotransporter Electrogenic 2 Deficiency." in: Hypertension, Vol. 66, Issue 1, pp. 68-74, (2015) (PubMed).

Cheng, Wu, Kortenoeven, Pisitkun, Fenton: "A Systems Level Analysis of Vasopressin-mediated Signaling Networks in Kidney Distal Convoluted Tubule Cells." in: Scientific reports, Vol. 5, pp. 12829, (2015) (PubMed).

Rosenbaek, Kortenoeven, Aroankins, Fenton: "Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis." in: The Journal of biological chemistry, Vol. 289, Issue 19, pp. 13347-61, (2014) (PubMed).

Esteva-Font, Guillén-Gómez, Diaz, Guirado, Facundo, Ars, Ballarin, Fernández-Llama: "Renal sodium transporters are increased in urinary exosomes of cyclosporine-treated kidney transplant patients." in: American journal of nephrology, Vol. 39, Issue 6, pp. 528-35, (2014) (PubMed).

Donnelly, Needham, Snyder, Roy, Khadem, Brodsky, Subramanya: "Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter endoplasmic reticulum-associated degradation and biogenesis." in: The Journal of biological chemistry, Vol. 288, Issue 18, pp. 13124-35, (2013) (PubMed).

Rosenbaek, Assentoft, Pedersen, MacAulay, Fenton: "Characterization of a novel phosphorylation site in the sodium-chloride cotransporter, NCC." in: The Journal of physiology, Vol. 590, Issue Pt 23, pp. 6121-39, (2012) (PubMed).

Target Details for SLC12A3

Target: SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))

Alternative Name: NCC

Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).

Gene ID: 54300, 6559

NCBI Accession: NP_062218, NP_000330

UniProt: P55018, P55017