HSPA4 antibody

Catalog No. ABIN863194

Product Details anti-HSPA4 Antibody

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Reactivity: Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~110 kDa.

Cross-Reactivity: Cow, Hamster, Human, Monkey, Mouse, Rat, Saccharomyces cerevisiae, Shark, Sheep

Purification: Protein A Purified

Immunogen: Synthetic peptide derived from the sequence of hamster HSP110, sequence identical to human and mouse

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN863193 was sufficient for detection of HSP110 in 10 μg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-HSPA4 antibody (ABIN863194)

Eng, Reed, Kokolus, Repasky: "Housing temperature influences the pattern of heat shock protein induction in mice following mild whole body hyperthermia." in: International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, Vol. 30, Issue 8, pp. 540-6, (2014) (PubMed).

Target Details for HSPA4

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Alternative Name: HSP110 (HSPA4 Products)

Synonyms: APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, Hsp110 antibody, Hsp70 antibody, irp94 antibody, 70kDa antibody, AI317151 antibody, Hsp70RY antibody, mKIAA4025 antibody, hspa4 antibody, wu:fi30e11 antibody, zgc:55743 antibody, zgc:77413 antibody, hs24/p52 antibody, hspa4-a antibody, osp94 antibody, pg-2 antibody, hspa4l antibody, wu:fc41d05 antibody, wu:fi59h02 antibody, wu:fj35c08 antibody, zgc:55506 antibody, heat shock protein family A (Hsp70) member 4 antibody, heat shock protein family A member 4 antibody, heat shock protein 4 antibody, heat shock protein 4b antibody, heat shock protein family A (Hsp70) member 4 S homeolog antibody, heat shock protein 4a antibody, HSPA4 antibody, Hspa4 antibody, hspa4b antibody, hspa4.S antibody, hspa4a antibody

Background: HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33 % amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).

Gene ID: 10808

NCBI Accession: NP_006635

UniProt: Q92599