DUX4 antibody (C-Term)

Catalog No. ABIN863109

Product Details anti-DUX4 Antibody

Target: DUX4 (Double Homeobox 4 (DUX4))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DUX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~45 kDa. No cross-reactivity with DUX4c.

Cross-Reactivity: Human, Mouse

Purification: Protein G Purified

Immunogen: C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag

Clone: P2B1

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN863109 was sufficient for detection of DUX4 in 20 μg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for DUX4

Target: DUX4 (Double Homeobox 4 (DUX4))

Alternative Name: DUX4 (DUX4 Products)

Synonyms: DUX4 antibody, DUX4L antibody, double homeobox, 4 antibody, double homeobox 4 antibody, DUX4 antibody

Background: DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain transcription factor 1 (1). Clinically it is a facioscapulohumeral muscular dystrophy candidate gene that appears to have a toxic gain of function (2-4). In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle and possibly other differentiated tissues (5).

Gene ID: 22947

NCBI Accession: NP_149418

UniProt: F5GZ66