LY75/DEC-205 antibody (PE)

Catalog No. ABIN807925

Product Details anti-LY75/DEC-205 Antibody (PE)

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LY75/DEC-205 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant Fc-tagged human CD205

Clone: HD30

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-LY75/DEC-205 antibody (PE) (ABIN807925)

Park, Rodriguez, Ueta, Lee, Pack, Matsuno, Steinman: "Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals." in: Journal of immunological methods, Vol. 377, Issue 1-2, pp. 15-22, (2012) (PubMed).

Target Details for LY75/DEC-205

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Alternative Name: CD205 (LY75 Products)

Synonyms: LY75 antibody, MGC140308 antibody, DEC205 antibody, im:7150926 antibody, si:dkey-78p20.2 antibody, LYT75 antibody, CD205 antibody, DEC-205 antibody, CLEC13B antibody, GP200-MR6 antibody, LY-75 antibody, RGD1565962 antibody, Ly-75 antibody, lymphocyte antigen 75 antibody, CD205 protein antibody, CD302 antigen antibody, LY75 antibody, Cyan7822_2917 antibody, LY5 antibody, ly75 antibody, cd205 antibody, LOC100380704 antibody, LOC100433861 antibody, LOC100483541 antibody, Ly75 antibody

Background: Lymphocyte antigen 75,CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.,DEC-205, LY75, CLEC13B

Gene ID: 4065

UniProt: O60449