TRMT12 antibody (N-Term)

Catalog No. ABIN783482

Product Details anti-TRMT12 Antibody

Target: TRMT12 (tRNA Methyltransferase 12 Homolog (TRMT12))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRMT12 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects TRMT12 at N-term. TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist, this antibody will detect both isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Affinity chromatography purified via peptide column

Immunogen: 19 amino acid peptide near the amino terminus of human TYW2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Target Details for TRMT12

Target: TRMT12 (tRNA Methyltransferase 12 Homolog (TRMT12))

Alternative Name: TRMT12 (TRMT12 Products)

Synonyms: 4632406N01Rik antibody, AI449275 antibody, Tyw2 antibody, TRM12 antibody, TYW2 antibody, RGD1307127 antibody, trmt12 antibody, tRNA methyltranferase 12 antibody, tRNA methyltransferase 12 homolog antibody, tRNA methyltransferase 12 antibody, tRNA methyltransferase 12 homolog (S. cerevisiae) antibody, Trmt12 antibody, TRMT12 antibody, trmt12 antibody

Background: TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.Synonyms: TRM12, TYW2, tRNA methyltransferase 12, tRNA wybutosine-synthesizing protein 2 homolog, tRNA-yW-synthesizing protein 2

Gene ID: 55039

NCBI Accession: NP_060426

UniProt: Q53H54