TRMT12 antibody (N-Term)

Catalog No. ABIN783482

Product Details anti-TRMT12 Antibody

Target: TRMT12 (tRNA Methyltransferase 12 Homolog (TRMT12))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRMT12 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects TRMT12 at N-term. TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist, this antibody will detect both isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Affinity chromatography purified via peptide column

Immunogen: 19 amino acid peptide near the amino terminus of human TYW2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Target Details for TRMT12

Target: TRMT12 (tRNA Methyltransferase 12 Homolog (TRMT12))

Alternative Name: TRMT12 (TRMT12 Products)

Background: TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.Synonyms: TRM12, TYW2, tRNA methyltransferase 12, tRNA wybutosine-synthesizing protein 2 homolog, tRNA-yW-synthesizing protein 2

Gene ID: 55039

NCBI Accession: NP_060426

UniProt: Q53H54