This antibody detects ZBTB4 (C-term). At least four isoforms of ZBTB4 are known to exist, this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.
Cross-Reactivity (Details)
Species reactivity (tested):Human, mouse
Purification
Affinity chromatography purified via peptide column
Immunogen
18 amino acid peptide near the carboxy terminus of human ZBTB4
The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions, this degradation increases with DNA damage.Synonyms: KAISO-L1, KAISO-like zinc finger protein 1, KIAA1538, Zinc finger and BTB domain-containing protein 4