Caspase 8 antibody (AA 217-221)

Catalog No. ABIN783313

Product Details anti-Caspase 8 Antibody

Target: Caspase 8 (CASP8)

Binding Specificity: AA 217-221

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: S-E-S-Q-T

Specificity: This antibody detects endogenous level of total Caspase 8 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Affinity-chromatography using epitope-specific peptide

Immunogen: Peptide sequence around aa.217~221 derived from Caspase 8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8 (CASP8 Products)

Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog

Molecular Weight: 57/45 kDa

Gene ID: 841

NCBI Accession: NP_001073593

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades