Synaptojanin 1 antibody (C-Term)

Catalog No. ABIN782444

Product Details anti-Synaptojanin 1 Antibody

Target: Synaptojanin 1 (SYNJ1)

Binding Specificity: C-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Synaptojanin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specific for the ~145k Synaptojanin I protein in Western blots of Rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody. Does not cross react with Mouse.

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Protein G Chromatography of Culture Supernatant.

Immunogen: Recombinant protein corresponding to amino acid residues from the C-terminal region of Rat Synaptojanin 1.

Clone: 5H1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for Synaptojanin 1

Target: Synaptojanin 1 (SYNJ1)

Alternative Name: Synaptojanin-1 / SYNJ1 (SYNJ1 Products)

Background: Synaptojanin is a phosphatidylinositol phosphatase involved in clathrinmediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms, one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996). The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down's syndrome (DS). It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidyl-inositol-4,5-bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008).Synonyms: 4, 5-trisphosphate 5-phosphatase 1, INPP5G, KIAA0910, Synaptic Marker, Synaptic inositol-1

Gene ID: 85238

NCBI Accession: NP_445928

UniProt: Q62910

Pathways: Inositol Metabolic Process, Synaptic Vesicle Exocytosis