FTO antibody (N-Term)

Catalog No. ABIN782440

Product Details anti-FTO Antibody

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: Specific for the ~58 kDa Fto protein in Western blots of rat testes lysate. The antibody has also been used for Immunocytochemistry with neuronal progenitor cells.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Horse, non-Human Primate and Sheep.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Protein G Chromatography of Culture Supernatant

Immunogen: Synthetic peptide -KLH conjugated corresponding to amino acid residues from the N- terminal region of FTO

Clone: 5-2H10

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Alternative Name: FTO (FTO Products)

Background: The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009).Synonyms: Fat mass and obesity-associated protein, KIAA1752

Gene ID: 79068

NCBI Accession: NP_001073901

UniProt: Q9C0B1