His Tag antibody
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- Target See all His Tag Antibodies
- His Tag
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This His Tag antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- HHHHHH
- Purification
- Affinity chromatography
- Clone
- 16-001
- Isotype
- IgG1
- Top Product
- Discover our top product His Tag Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS pH 7.4, with 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Upon receipt, store (in aliquots) at -20 to -70 °C.
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- Target
- His Tag
- Alternative Name
- 6xHistidine Epitope Tag (His Tag Products)
- Target Type
- Tag
- Background
- 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
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