CD163 antibody (PE)

Catalog No. ABIN775717

Product Details anti-CD163 Antibody (PE)

Target: CD163

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody GHI/61 recognizes an extracellular epitope CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Hairy cell leukemia cells

Clone: GHI-61

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD163 antibody (PE) (ABIN775717)

Kusi, Gyan, Goka, Dodoo, Obeng-Adjei, Troye-Blomberg, Akanmori, Adjimani: "Levels of soluble CD163 and severity of malaria in children in Ghana." in: Clinical and vaccine immunology : CVI, Vol. 15, Issue 9, pp. 1456-60, (2008) (PubMed).

Moniuszko, Kowal, Rusak, Pietruczuk, Dabrowska, Bodzenta-Lukaszyk: "Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants." in: Clinical and vaccine immunology : CVI, Vol. 13, Issue 6, pp. 704-7, (2006) (PubMed).

Kim, Alvarez, Fisher, Bronfin, Westmoreland, McLaurin, Williams: "CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood." in: The American journal of pathology, Vol. 168, Issue 3, pp. 822-34, (2006) (PubMed).

Law, Micklem, Shaw, Zhang, Dong, Willis, Mason: "A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily." in: European journal of immunology, Vol. 23, Issue 9, pp. 2320-5, (1993) (PubMed).

Pulford, Micklem, McCarthy, Cordell, Jones, Mason: "A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4." in: Immunology, Vol. 75, Issue 4, pp. 588-95, (1992) (PubMed).

Target Details for CD163

Target: CD163

Alternative Name: CD163 (CD163 Products)

Synonyms: DMBT1 antibody, CD163 antibody, CD163v2 antibody, CD163v3 antibody, M130 antibody, MM130 antibody, deleted in malignant brain tumors 1 protein-like antibody, CD163 molecule antibody, CD163 antigen antibody, LOC426820 antibody, CD163 antibody, Cd163 antibody

Background: CD163 Molecule,CD163, also known as M130, is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.,MM130, SCARI1

Gene ID: 9332

UniProt: Q86VB7