NOTCH4 antibody (AA 1261-1273)

Catalog No. ABIN768617

Product Details anti-NOTCH4 Antibody

Target: NOTCH4 (Notch 4 (NOTCH4))

Binding Specificity: AA 1261-1273

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NOTCH4 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Purpose: NOTCH4 (aa1261-1273)

Sequence: DHFHNGHCEK GCNN

Specificity: The immunizong peptide represents part of the extracellular domain.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence DHFHNGHCEKGCNN, from the internal region of the protein sequence according to NP_004548.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 27 kDa band in all human, mouse and rat tissue and cell line lysates tested after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we ob

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and membranes of HeLa cells, and in the membranes of NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NOTCH4

Target: NOTCH4 (Notch 4 (NOTCH4))

Alternative Name: NOTCH4 (NOTCH4 Products)

Synonyms: INT3 antibody, NOTCH3 antibody, Int-3 antibody, Int3 antibody, N4 antibody, Notch4 antibody, notch 4 antibody, neurogenic locus notch homolog protein 4 antibody, NOTCH4 antibody, Notch4 antibody, LOC100712850 antibody

Background: NOTCH4, DAQB-143M3.4, FLJ16302, INT3, MGC74442, NOTCH3, Notch homolog 4, neurogenic locus notch homolog protein 4

Molecular Weight: 27kDa

Gene ID: 4855, 18132, 406162

NCBI Accession: NP_004548

Pathways: Notch Signaling