PROL1 antibody (Internal Region)

Catalog No. ABIN768578

Product Details anti-PROL1 Antibody

Target: PROL1 (Proline Rich, Lacrimal 1 (PROL1))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PROL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

Purpose: Mucin 10 / Prol1

Sequence: QFPVRKYLED PRY

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QFPVRKYLEDPRY, from the internal region of the protein sequence according to NP_032670.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Positive staining in the submandibular salivary gland of the mouse, while cells remain negative in the sublingual salivary gland. Data provided by Everest Grant winner Melinda Larsen State University of New York, Albany, NY. A publi

Western Blot: This antibody has been successfully used in WB on Mouse: Peluso G et al. (2019) J Biol Chem. 2019 Dec 27. pii: jbc.RA119.009807. PMID: 31882545.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: This antibody has been successfully used in IF on Mouse: Peluso G et al. (2019) J Biol Chem. 2019 Dec 27. pii: jbc.RA119.009807. PMID: 31882545.

Immunoprecipitation: This antibody has been successfu

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PROL1 antibody (ABIN768578)

Tanaka, Ogawa, Hojo, Kawashima, Mabuchi, Hata, Nakamura, Yasuhara, Takamatsu, Irié, Fukada, Sakai, Inoue, Nishimura, Ohara, Saito, Ohba, Tsuji, Mishima: "Generation of orthotopically functional salivary gland from embryonic stem cells." in: Nature communications, Vol. 9, Issue 1, pp. 4216, (2019) (PubMed).

Nelson, Manhardt, Kamath, Sui, Santamaria-Pang, Can, Bello, Corwin, Dinn, Lazare, Gervais, Sequeira, Peters, Ginty, Gerdes, Larsen: "Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation." in: Biology open, Vol. 2, Issue 5, pp. 439-47, (2013) (PubMed).

Target Details for PROL1

Target: PROL1 (Proline Rich, Lacrimal 1 (PROL1))

Alternative Name: Prol1 (PROL1 Products)

Synonyms: BPLP antibody, PRL1 antibody, opiorphin antibody, Muc10 antibody, opiorphin prepropeptide antibody, proline rich, lacrimal 1 antibody, OPRPN antibody, Prol1 antibody

Background: Prol1, proline rich, lacrimal 1, Muc10, mucin 10, mucin 10, submandibular gland salivary mucin

Molecular Weight: 45kDa

Gene ID: 17830

NCBI Accession: NP_032670