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SIRT3 antibody (Internal Region)

Verified SIRT3 Reactivity: Mouse WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN768568
  • Target See all SIRT3 Antibodies
    SIRT3 (Sirtuin 3 (SIRT3))
    Binding Specificity
    • 17
    • 10
    • 8
    • 7
    • 7
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 94
    • 40
    • 18
    • 9
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    Mouse
    Host
    • 83
    • 15
    • 2
    • 2
    • 1
    Goat
    Clonality
    • 90
    • 14
    Polyclonal
    Conjugate
    • 55
    • 15
    • 12
    • 6
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SIRT3 antibody is un-conjugated
    Application
    • 91
    • 61
    • 41
    • 10
    • 8
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    Sirtuin 3 (mouse)
    Sequence
    RASGIPASKL VEAH
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_071878.2, NP_001171275.1). Reported variants represent identical proteins: NP_071878.2, NP_001120823.1
    Cross-Reactivity
    Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-RASGIPASKLVEAH, from the internal region of the protein sequence according to NP_071878.2, NP_001171275.1.
    Isotype
    IgG
    Top Product
    Discover our top product SIRT3 Primary Antibody
  • Application Notes

    Western Blot: Approx 30 kDa band observed in Mouse Heart lysates (calculated MW of 28.8 kDa according to NP_071878.2). An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    SIRT3 (Sirtuin 3 (SIRT3))
    Alternative Name
    Sirt3 (SIRT3 Products)
    Synonyms
    zgc:158774 antibody, sirtuin antibody, SIR2L3 antibody, 2310003L23Rik antibody, AI848213 antibody, Sir2l3 antibody, sirtuin 3 antibody, sirt3 antibody, SIRT3 antibody, Sirt3 antibody
    Background
    Sirt3, sirtuin 3, sirtuin 3 (silent mating type information regulation 2, homolog) 3, 2310003L23Rik, AI848213, Sir2l3, NAD-dependent deacetylase sirtuin-3, OTTMUSP00000027441, OTTMUSP00000027442, OTTMUSP00000027444, OTTMUSP00000027445, SIR2-like protein 3
    Molecular Weight
    28.8kDa according to NP_071878.2
    Gene ID
    23410, 64384, 293615
    NCBI Accession
    NP_071878, NP_001171275
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