SIRT3 antibody (Internal Region)

Catalog No. ABIN768568

Product Details anti-SIRT3 Antibody

Target: SIRT3 (Sirtuin 3 (SIRT3))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Sirtuin 3 (mouse)

Sequence: RASGIPASKL VEAH

Specificity: This antibody is expected to recognize both reported isoforms (NP_071878.2, NP_001171275.1). Reported variants represent identical proteins: NP_071878.2, NP_001120823.1

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RASGIPASKLVEAH, from the internal region of the protein sequence according to NP_071878.2, NP_001171275.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Mouse Heart lysates (calculated MW of 28.8 kDa according to NP_071878.2). An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: Sirt3 (SIRT3 Products)

Synonyms: zgc:158774 antibody, sirtuin antibody, SIR2L3 antibody, 2310003L23Rik antibody, AI848213 antibody, Sir2l3 antibody, sirtuin 3 antibody, sirt3 antibody, SIRT3 antibody, Sirt3 antibody

Background: Sirt3, sirtuin 3, sirtuin 3 (silent mating type information regulation 2, homolog) 3, 2310003L23Rik, AI848213, Sir2l3, NAD-dependent deacetylase sirtuin-3, OTTMUSP00000027441, OTTMUSP00000027442, OTTMUSP00000027444, OTTMUSP00000027445, SIR2-like protein 3

Molecular Weight: 28.8kDa according to NP_071878.2

Gene ID: 23410, 64384, 293615

NCBI Accession: NP_071878, NP_001171275