AES antibody (N-Term)

Catalog No. ABIN768545

Product Details anti-AES Antibody

Target: AES (Amino-terminal Enhancer of Split (AES))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AES antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: AES

Sequence: SHLPQQLKFT TSD

Specificity: This antibody is expected to recognize all reported isoforms (NP_945320.1, NP_001121.2, NP_945321.1).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence SHLPQQLKFTTSD-C, from the N Terminus of the protein sequence according to NP_945320.1, NP_001121.2, NP_945321.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 30 kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 29.1 kDa according to NP_945320.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AES

Target: AES (Amino-terminal Enhancer of Split (AES))

Alternative Name: AES (AES Products)

Synonyms: AES-1 antibody, AES-2 antibody, ESP1 antibody, GRG antibody, GRG5 antibody, TLE5 antibody, AL024115 antibody, Esp1 antibody, Grg antibody, Grg-5 antibody, Grg5 antibody, Tle5 antibody, zgc:64216 antibody, aes-1 antibody, aes-2 antibody, esp1 antibody, grg antibody, grg5 antibody, tle5 antibody, xgrg-5 antibody, AES antibody, amino-terminal enhancer of split antibody, amino-terminal enhancer of split S homeolog antibody, AES antibody, Aes antibody, aes antibody, aes.S antibody

Background: AES, amino-terminal enhancer of split, AES-1, AES-2, ESP1, GRG, GRG5, TLE5, amino enhancer of split, gp130-associated protein GAM

Molecular Weight: 29.1kDa according to NP_945320.1

Gene ID: 166, 14797, 29466

NCBI Accession: NP_945320, NP_001121, NP_945321

Pathways: WNT Signaling, SARS-CoV-2 Protein Interactome