C22orf28 antibody (AA 201-215)

Catalog No. ABIN768540

Product Details anti-C22orf28 Antibody

Target: C22orf28 (Chromosome 22 Open Reading Frame 28 (C22orf28))

Binding Specificity: AA 201-215

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This C22orf28 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: HSPC117 (aa201-215)

Sequence: QADPNKVSAR AKKR

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QADPNKVSARAKKR, from the internal region of the protein sequence according to NP_055121.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear and vicinity staining in spermatogonia. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 55 kDa band observed in lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 55.2 kDa according to NP_055121.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for C22orf28

Target: C22orf28 (Chromosome 22 Open Reading Frame 28 (C22orf28))

Alternative Name: C22orf28 (C22orf28 Products)

Synonyms: C1H22orf28 antibody, C22orf28 antibody, DJ149A16.6 antibody, FAAP antibody, RP1-149A16.6 antibody, c22orf28 antibody, C10H22orf28 antibody, MGC154502 antibody, HSPC117 antibody, AI255213 antibody, AI463255 antibody, RNA 2',3'-cyclic phosphate and 5'-OH ligase antibody, tRNA-splicing ligase RtcB homolog antibody, RTCB antibody, rtcb antibody, rtcb.L antibody, Rtcb antibody

Background: C22orf28, chromosome 22 open reading frame 28, DJ149A16.6, RP1-149A16.6, hypothetical protein LOC51493, novel protein HSPC117

Molecular Weight: 55.2kDa according to NP_055121.1

Gene ID: 51493, 28088, 362855

NCBI Accession: NP_055121