SIRT7 antibody (AA 231-330)

Catalog No. ABIN760571

Product Details anti-SIRT7 Antibody

Target: SIRT7 (Sirtuin 7 (SIRT7))

Binding Specificity: AA 231-330

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SIRT7

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SIRT7 antibody (ABIN760571)

Wronska, Lawniczak, Wierzbicki, Kmiec: "Age-Related Changes in Sirtuin 7 Expression in Calorie-Restricted and Refed Rats." in: Gerontology, Vol. 62, Issue 3, pp. 304-10, (2016) (PubMed).

Takumida, Takumida, Anniko: "Localization of sirtuins in the mouse inner ear." in: Acta oto-laryngologica, Vol. 134, Issue 4, pp. 331-8, (2014) (PubMed).

Target Details for SIRT7

Target: SIRT7 (Sirtuin 7 (SIRT7))

Alternative Name: Sirt7 (SIRT7 Products)

Synonyms: SIRT7 antibody, LOC796135 antibody, SIR2L7 antibody, sirtuin 7 antibody, phosphate cytidylyltransferase 2, ethanolamine antibody, SIRT7 antibody, sirt7 antibody, PCYT2 antibody, Sirt7 antibody

Background:

Synonyms: SIR2L7, NAD-dependent protein deacetylase sirtuin-7, Regulatory protein SIR2 homolog 7, SIR2-like protein 7, SIRT7

Background: NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.

Gene ID: 51547

UniProt: Q9NRC8