CTCFL antibody (AA 351-450)

Catalog No. ABIN760421

Product Details anti-CTCFL Antibody

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Binding Specificity: AA 351-450

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTCFL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BORIS/CT27

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CTCFL

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Alternative Name: Ct27 (CTCFL Products)

Synonyms: BORIS antibody, CT27 antibody, CTCF-T antibody, HMGB1L1 antibody, dJ579F20.2 antibody, CTCFL antibody, Boris antibody, OTTMUSG00000016680 antibody, CCCTC-binding factor like antibody, CCCTC-binding factor (zinc finger protein)-like antibody, CTCFL antibody, Ctcfl antibody

Background:

Synonyms: BORIS like protein, Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CCCTC binding factor zinc finger protein like, CCCTC-binding factor, CT27, CTCF paralog, CTCF T, CTCF-like protein, CTCFL, CTCFL_HUMAN, HMG 1L1, HMGB1L1, Putative high mobility group protein 1 like 1, Putative high mobility group protein B1 like 1, Transcriptional repressor CTCFL, Zinc finger protein CTCF-T.

Background: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.

Gene ID: 140690