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CDC25B antibody (pSer186)

CDC25B Reactivity: Mouse WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN756967
  • Target See all CDC25B Antibodies
    CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
    Binding Specificity
    • 28
    • 26
    • 23
    • 15
    • 14
    • 12
    • 9
    • 9
    • 8
    • 8
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer186
    Reactivity
    • 145
    • 116
    • 55
    • 7
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    Mouse
    Host
    • 198
    • 2
    Rabbit
    Clonality
    • 189
    • 11
    Polyclonal
    Conjugate
    • 73
    • 15
    • 12
    • 11
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    This CDC25B antibody is un-conjugated
    Application
    • 169
    • 75
    • 51
    • 51
    • 36
    • 30
    • 16
    • 11
    • 5
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity
    Mouse
    Predicted Reactivity
    Human,Rat
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser186
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
    Alternative Name
    Cdc25B (CDC25B Products)
    Synonyms
    CDC25B antibody, AI604853 antibody, cell division cycle 25B antibody, cdc25b antibody, CDC25B antibody, Cdc25b antibody
    Background

    Synonyms: M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2

    Background: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

    Gene ID
    994
    UniProt
    P30305
    Pathways
    Cell Division Cycle, M Phase, Autophagy
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