SNRPE antibody (pSer446)

Catalog No. ABIN756787

Product Details anti-SNRPE Antibody

Target: SNRPE (Small Nuclear Ribonucleoprotein Polypeptide E (SNRPE))

Binding Specificity: pSer446

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNRPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Ser446

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SNRPE

Target: SNRPE (Small Nuclear Ribonucleoprotein Polypeptide E (SNRPE))

Alternative Name: B-Raf (SNRPE Products)

Synonyms: B-raf antibody, HYPT11 antibody, SME antibody, Sm-E antibody, b-raf antibody, sm-e antibody, sme antibody, SmE antibody, snRNP-E antibody, AL022645 antibody, C76690 antibody, zgc:56682 antibody, small nuclear ribonucleoprotein polypeptide E antibody, small nuclear ribonucleoprotein polypeptide E L homeolog antibody, small nuclear ribonucleoprotein E antibody, SNRPE antibody, snrpe.L antibody, Snrpe antibody, snrpe antibody

Background:

Synonyms: B Raf phospho S446, p-B Raf phospho S446, 94 kDa B raf protein, B raf 1, B Raf proto oncogene serine threonine protein kinase, BRAF 1, Braf, BRAF1, cRmil, MGC126806, MGC138284, Murine sarcoma viral v-raf oncogene homolog B1, Murine sarcoma viral v raf oncogene homolog B1, p94, RAFB 1, RAFB1, v raf murine sarcoma viral oncogene homolog B1, FLJ95109.

Background: The Raf kinases are important intermediates in signal transduction. Raf protein family members, including A Raf and B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase). Defects in BRAF are involved in a wide range of cancers. B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. 1 BRAF is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. BRAF acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones.

Gene ID: 673

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C