AKT 1/2/3 antibody (pThr305, pThr308, pThr309)

Catalog No. ABIN756052

Product Details anti-AKT 1/2/3 Antibody

Target: AKT 1/2/3

Binding Specificity: pThr305, pThr308, pThr309

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT 1/2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The antibody recognizes phosphorylation at Thr308 of AKT1 and because of sequence similarity, it will also detect phosphorylation of Thr309 of AKT2 and phosphorylation at Thr305 of AKT3.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Thr308

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for AKT 1/2/3

Target: AKT 1/2/3

Alternative Name: AKT1/2/3 (AKT 1/2/3 Products)

Background:

Synonyms: AKT1 phospho T308, AKT2 phospho T309, AKT3 phospho T305,p-AKT1 phospho T308, p-AKT2 phospho T309, p-AKT3 phospho T305, AKT 1, AKT, AKT1, AKT-1, AKT1_HUMAN, AKT 2, AKT2, AKT-2, AKT2_HUMAN, AKT 3, AKT3, AKT-3, AKT3_HUMAN, C AKT, cAKT, MGC9965, MGC99656, Oncogene AKT1, PKB, PKB alpha, PKB-ALPHA, PKB beta, PKB gamma, PRKBA, Protein Kinase B Alpha, Protein kinase B, Proto-oncogene c-Akt, RAC Alpha, RAC alpha serine/threonine protein kinase, RAC, RAC PK Alpha, Rac protein kinase alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, v akt murine thymoma viral oncogene homolog 1, vAKT Murine Thymoma Viral Oncogene Homolog 1.

Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

Gene ID: 207

UniProt: Q9Y243