NCEH1 antibody (AA 151-250)

Catalog No. ABIN755752

Product Details anti-NCEH1 Antibody

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Binding Specificity: AA 151-250

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCEH1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AADACL1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NCEH1

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Alternative Name: AADACL1 (NCEH1 Products)

Synonyms: AADACL1 antibody, NCEH antibody, Aadacl1 antibody, B230106I24Rik antibody, CPO-BP antibody, Nceh antibody, RGD1311104 antibody, nceh antibody, aadacl1 antibody, MGC79479 antibody, nceh1b antibody, zgc:153038 antibody, zgc:92416 antibody, neutral cholesterol ester hydrolase 1 antibody, neutral cholesterol ester hydrolase 1 S homeolog antibody, neutral cholesterol ester hydrolase 1b, tandem duplicate 1 antibody, neutral cholesterol ester hydrolase 1a antibody, NCEH1 antibody, Nceh1 antibody, nceh1 antibody, nceh1.S antibody, nceh1b.1 antibody, nceh1a antibody

Background:

Synonyms: Arylacetamide deacetylase like 1, Arylacetamide deacetylase-like 1, Kiaa1363, NCEH, Nceh1, NCEH1_HUMAN, Neutral cholesterol ester hydrolase 1, Neutral cholesterol ester hydrolase.

Background: The assembly of very-low-density lipoproteins (VLDLs) in the secretory apparatus of the hepatocyte relies on the mobilization of triacylglycerol (TAG) from the cytosolic pool by lipolysis and re-esterification. However, some of the re-esterified TAG products are returned to the cytosolic pool in the liver, which protects vulnerable body tissues from excess lipotoxic non-esterified fatty acids in the plasma. Some of the lipases involved in this process include arylacetamide deacetylase (AADAC) and its related proteins AADACL1 and AADACL2. AADAC, a single pass type II membrane protein of the endoplasmic reticulum, is expressed in hepatocytes, intestinal mucosal cells, pancreas and adrenal gland. It plays an important role in the metabolic activation of arylamine substrates to ultimate carcinogens. AADACL1 hydrolyzes the metabolic intermediate 2-acetyl monoalkylglycerol, and its inactivation results in disruption of ether lipid metabolism in cancer cells and impaired cell migration and tumor growth.