TRIM25 antibody (AA 531-630)

Catalog No. ABIN754858

Product Details anti-TRIM25 Antibody

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Binding Specificity: AA 531-630

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TRIM25

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25 (TRIM25 Products)

Synonyms: zgc:55773 antibody, MGC130751 antibody, EFP antibody, RNF147 antibody, Z147 antibody, ZNF147 antibody, AA960166 antibody, AL022677 antibody, Zfp147 antibody, Efp antibody, Znf147 antibody, E3 ubiquitin/ISG15 ligase TRIM25 antibody, tripartite motif containing 25 antibody, E3 ubiquitin/ISG15 ligase TRIM25-like L homeolog antibody, microRNA 3614 antibody, tripartite motif-containing 25 antibody, LOC100146440 antibody, trim25 antibody, LOC100490741.L antibody, TRIM25 antibody, MIR3614 antibody, Trim25 antibody

Background:

Synonyms: EFP, Z147, RNF147, ZNF147, E3 ubiquitin/ISG15 ligase TRIM25, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25

Background: Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

Gene ID: 7706

UniProt: Q14258