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ATG12 antibody (AA 101-187)

Name: ATG12 antibody (AA 101-187)
SKU: ABIN752518
Price: 384.62 USD
Availability: InStock

ATG12 Reactivity: Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse Kidney tissue labeled with Anti Apg12 Polyclonal Antibody, unconjugated (ABIN752518) at 1:200 followed by incubation with conjugated secondary antibody and DAB staining. (ATG12 antibody (AA 101-187))

IHC

Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti Apg12 Polyclonal Antibody,Unconjugated (ABIN752518) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (ATG12 antibody (AA 101-187))

IHC

Formalin-fixed and paraffin embedded rat lung labeled with Rabbit Anti-Apg12 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (ATG12 antibody (AA 101-187))

IHC (p)

3 images

(1 reference)

Catalog No. ABIN752518

Datasheet as PDF

Target See all ATG12 Antibodies

ATG12 (Autophagy Related 12 (ATG12))
Binding Specificity
14

13

8

7

6

6

6

3

2

2

2

1

1

1

1

1

1

1

1
AA 101-187
Reactivity
77

35

25

6

6

6

6

5

4

4

4

4

2

2

2

2

2

2

2

2

1
Mouse, Rat
Host
73

19

2
Rabbit
Compatible Secondaries
Clonality
73

21
Polyclonal
Conjugate
46

7

6

6

6

4

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATG12 antibody is un-conjugated
Application
72

39

30

28

21

12

12

9

8

3

2

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Mouse, Rat

Predicted Reactivity

Human,Dog,Cow,Pig,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human ATG12

Isotype

IgG

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ATG12 Reactivity: Human WB, ELISA, IHC, IF Host: Mouse Monoclonal 43CT73-3-5-5-4 unconjugated

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ATG12 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

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ATG12 Reactivity: Human, Mouse WB, ELISA, IF Host: Rabbit Polyclonal unconjugated

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ATG12 Reactivity: Human, Mouse IF Host: Rabbit Polyclonal RB2793 unconjugated

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ATG12 Reactivity: Human WB Host: Mouse Monoclonal 43CT73-3-5-5-4 unconjugated

anti-Autophagy Related 12 (ATG12) (N-Term) antibody (HRP)
ATG12 Reactivity: Human WB, IF, ICC Host: Rabbit Polyclonal HRP

anti-Autophagy Related 12 (ATG12) (N-Term) antibody (FITC)
ATG12 Reactivity: Human WB, IF, ICC Host: Rabbit Polyclonal FITC

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Song, Kou, Zou, Gao, Zeng, Xie: "Involvement of autophagy in tri-ortho-cresyl phosphate- induced delayed neuropathy in hens." in: Neurochemistry international, Vol. 64, pp. 1-8, (2013) (PubMed).
Target

ATG12 (Autophagy Related 12 (ATG12))

Alternative Name

Apg12 (ATG12 Products)

Background

Synonyms: APG12, FBR93, APG12L, HAPG12, Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, ATG12
Background: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG1 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.

Gene ID

9140

UniProt

O94817

Pathways

Autophagy