ATG12 antibody (AA 101-187)

Catalog No. ABIN752518

Product Details anti-ATG12 Antibody

Target: ATG12 (Autophagy Related 12 (ATG12))

Binding Specificity: AA 101-187

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATG12

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ATG12 antibody (ABIN752518)

Song, Kou, Zou, Gao, Zeng, Xie: "Involvement of autophagy in tri-ortho-cresyl phosphate- induced delayed neuropathy in hens." in: Neurochemistry international, Vol. 64, pp. 1-8, (2013) (PubMed).

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: Apg12 (ATG12 Products)

Synonyms: APG12 antibody, APG12L antibody, FBR93 antibody, HAPG12 antibody, Apg12l antibody, AUTOPHAGY 12 antibody, AUTOPHAGY 12 B antibody, CG10861 antibody, Dmel\\CG10861 antibody, atg12 antibody, ATG12 antibody, apg12 antibody, DDBDRAFT_0185323 antibody, DDBDRAFT_0191412 antibody, DDB_0185323 antibody, DDB_0191412 antibody, 4931423H11Rik antibody, A330058M13Rik antibody, Atg12l antibody, AO090001000593 antibody, autophagy related 12 antibody, Ubiquitin-like superfamily protein antibody, Atg12p antibody, autophagy protein antibody, ubiquitin-like protein ATG12B antibody, autophagy associated protein Atg12 antibody, Autophagy-related 12 antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene antibody, autophagy associated ubiquitin-like modifier Atg12 antibody, hypothetical protein antibody, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) antibody, autophagy protein 12 antibody, autophagy related 12 S homeolog antibody, autophagy-related protein 12 antibody, ATG12 antibody, Atg12 antibody, APG12B antibody, APG12 antibody, LOC9321066 antibody, apg12 antibody, atg12 antibody, LOC743425 antibody, CAALFM_C113950CA antibody, atg12.S antibody, ANI_1_922094 antibody, AOR_1_1064164 antibody

Background:

Synonyms: APG12, FBR93, APG12L, HAPG12, Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, ATG12

Background: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG1 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.

Gene ID: 9140

UniProt: O94817

Pathways: Autophagy