PRKAA1/PRKAA2 antibody (pThr172, pThr183)

Catalog No. ABIN752368

Product Details anti-PRKAA1/PRKAA2 Antibody

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Binding Specificity: pThr172, pThr183

Reactivity: Human, Mouse, Rat, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1/PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Pig, Rat, Sheep

Predicted Reactivity: Dog,Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse AMPK alpha 2 around the phosphorylation site of Thr172

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PRKAA1/PRKAA2 antibody (ABIN752368)

Saito, Chikenji, Ozasa, Fujimiya, Yamashita, Gingery, Iba: "PDGFR Signaling Mediates Hyperproliferation and Fibrotic Responses of Subsynovial Connective Tissue Cells in Idiopathic Carpal Tunnel Syndrome." in: Scientific reports, Vol. 7, Issue 1, pp. 16192, (2019) (PubMed).

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Wang, Zhao, Yu, Feng, Zhang, Kou, Chu, Cui, Li, Zhang, Shen, Min: "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." in: Toxicology and applied pharmacology, Vol. 313, pp. 149-158, (2016) (PubMed).

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, (2016) (PubMed).

Target Details for PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Alternative Name: AMPK alpha-1/2 (PRKAA1/PRKAA2 Products)

Synonyms: AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, PRKAA2 antibody, Prkaa2 antibody

Background:

Synonyms: PRKAA1phospho T172, AMPK alpha 1 + AMPK alpha 2 phospho T183/T172, 5 AMP activated protein kinase alpha 1catalytic subunit, 5 AMP activated protein kinase catalytic alpha 1 chain, 5' AMP activated protein kinase catalytic subunit alpha 1, AAPK1, acetyl CoA carboxylase kinase, AI194361, AI450832, AL024255, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha -1, AMPK 1, AMPK alpha 1 chain, AMPK, AMPK1, AMPKa1, AMPKalpha1, C130083N04Rik, cb116, EC 2.7.11.1, HMG CoA reductase kinase,hormone sensitive lipase kinase, im:7154392, kinase AMPK alpha1, MGC33776, MGC57364, PRKAA 1, PRKAA1, Protein kinase AMP activated alpha 1 catalytic subunit, SNF1-like protein AMPK, wu:fa94C10.

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].

Gene ID: 5562