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NCF1 antibody (AA 151-250)

Name: NCF1 antibody (AA 151-250)
SKU: ABIN750643
Price: 384.62 USD
Availability: InStock

NCF1 Reactivity: Human, Mouse, Horse WB, ELISA, IHC (p), IF (cc), FACS Host: Rabbit Polyclonal unconjugated

Mouse bone, lymph node, and spleen lysates probed with Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (NCF1 antibody (AA 151-250))

Lane 1: human colon carcinoma lysates Lane 2: mouse brain lysates probed with Anti NCF1/p47 phox Polyclonal Antibody, Unconjugated (ABIN750643) at 1:200 in 4 °C. (NCF1 antibody (AA 151-250))

SDS

Formalin-fixed and human endometrium tissue labeled with Anti-NCF1/p47 phox Polyclonal Antibody, Unconjugated (ABIN750643) at 1:100 followed by conjugation to the secondary antibody and DAB staining (NCF1 antibody (AA 151-250))

IHC

Formalin-fixed and human endometrium tissue labeled with Anti-NCF1/p47 phox Polyclonal Antibody, Unconjugated at 1:100 followed by conjugation to the secondary antibody and DAB staining (NCF1 antibody (AA 151-250))

IHC (p)

4 images

Catalog No. ABIN750643

Datasheet as PDF

Target See all NCF1 Antibodies

NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
Binding Specificity
28

15

13

11

10

8

7

6

6

6

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 151-250
Reactivity
127

48

44

15

8

4

3

3

3

3
Human, Mouse, Horse
Host
139

2

1
Rabbit
Compatible Secondaries
Clonality
140

1
Polyclonal
Conjugate
72

8

6

5

4

4

4

4

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2
This NCF1 antibody is un-conjugated
Application
103

65

61

25

24

16

14

13

8

8

4

4

3
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Cross-Reactivity

Horse, Human, Mouse

Predicted Reactivity

Rat,Dog

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human NCF1

Isotype

IgG

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anti-Neutrophil Cytosol Factor 1 (NCF1) (C-Term) antibody
NCF1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

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NCF1 Reactivity: Human, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

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NCF1 Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Neutrophil Cytosol Factor 1 (NCF1) (AA 1-390) antibody
NCF1 Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Neutrophil Cytosol Factor 1 (NCF1) (AA 331-380), (pSer359) antibody
NCF1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Neutrophil Cytosol Factor 1 (NCF1) (Ser345) antibody
NCF1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name

NCF1/p47 phox (NCF1 Products)

Synonyms

NCF1A antibody, NOXO2 antibody, SH3PXD1A antibody, p47phox antibody, Ncf-1 antibody, p47 antibody, neutrophil cytosolic factor 1 antibody, NCF1 antibody, Ncf1 antibody

Background

Synonyms: NCF1A, NOXO2, p47phox, SH3PXD1A, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1
Background: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Gene ID

653361

UniProt

P14598

Pathways

PI3K-Akt Signaling