CYBA antibody (AA 21-120)

Catalog No. ABIN750538

Product Details anti-CYBA Antibody

Target: CYBA (Cytochrome B-245, alpha Polypeptide (CYBA))

Binding Specificity: AA 21-120

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYBA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CK18 Cytochrome b245 Light Chain/p22phox

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CYBA antibody (ABIN750538)

Song, Zhang, Han, Zhang, Zhang, Gao, Zhang, Chu, Zhao: "Potential mechanisms underlying the protective effects of salvianic acid A against atherosclerosis in vivo and vitro." in: Biomedicine & pharmacotherapy, Vol. 109, pp. 945-956, (2019) (PubMed).

Yue, Wang, Wang, Du, Shen, Tang, Wang, Yin: "Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways." in: International journal of biological macromolecules, Vol. 89, pp. 376-88, (2016) (PubMed).

Yoshida, Murayama, Kawashima, Nagahara, Kangawa, Mizukami, Kimura, Abe, Hayashi, Shibutani: "Apocynin and enzymatically modified isoquercitrin suppress the expression of a NADPH oxidase subunit p22phox in steatosis-related preneoplastic liver foci of rats." in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol. 69, Issue 1, pp. 9-16, (2016) (PubMed).

Li, Wang, Zhu, Zhang, Pan, Wang et al.: "Arctigenin suppresses transforming growth factor-?1-induced expression of monocyte chemoattractant protein-1 and the subsequent epithelial-mesenchymal transition through reactive oxygen ..." in: Free radical research, Vol. 49, Issue 9, pp. 1095-113, (2015) (PubMed).

Walton, Shin, Tajinda, Heusner, Kogan, Miyake, Chen, Tamura, Matsumoto: "Adult neurogenesis transiently generates oxidative stress." in: PLoS ONE, Vol. 7, Issue 4, pp. e35264, (2012) (PubMed).

Target Details for CYBA

Target: CYBA (Cytochrome B-245, alpha Polypeptide (CYBA))

Alternative Name: Cytochrome b245 Light Chain (CYBA Products)

Synonyms: zgc:66077 antibody, MGC80750 antibody, CYBA antibody, p22-phox antibody, p22phox antibody, p22-PHOX antibody, b558 antibody, nmf333 antibody, P22-PHOX antibody, cytochrome b-245, alpha polypeptide antibody, cytochrome b-245 alpha polypeptide L homeolog antibody, cytochrome b-245 alpha polypeptide antibody, cytochrome b-245 light chain-like antibody, cytochrome b-245 alpha chain antibody, p22-phox antibody, cyba antibody, cyba.L antibody, CYBA antibody, LOC101328418 antibody, Cyba antibody

Background:

Synonyms: p22-PHOX, Cytochrome b-245 light chain, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22phox, CYBA

Background: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

Gene ID: 1535

UniProt: P13498

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis