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S100A8 antibody (AA 25-89)

S100A8 Reactivity: Human, Mouse ELISA, FACS, IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN749273
  • Target See all S100A8 Antibodies
    S100A8 (S100 Calcium Binding Protein A8 (S100A8))
    Binding Specificity
    • 18
    • 18
    • 15
    • 9
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    AA 25-89
    Reactivity
    • 92
    • 50
    • 33
    • 14
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    Human, Mouse
    Host
    • 91
    • 36
    • 3
    • 3
    • 1
    Rabbit
    Clonality
    • 96
    • 38
    Polyclonal
    Conjugate
    • 61
    • 21
    • 17
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This S100A8 antibody is un-conjugated
    Application
    • 84
    • 48
    • 41
    • 28
    • 16
    • 14
    • 12
    • 12
    • 12
    • 11
    • 7
    • 6
    • 6
    • 6
    • 3
    • 3
    • 1
    ELISA, Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
    Cross-Reactivity
    Human, Mouse
    Predicted Reactivity
    Rat
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from mouse S100-A8
    Isotype
    IgG
    Top Product
    Discover our top product S100A8 Primary Antibody
  • Application Notes
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Nguyen, Fentress, Qiu, Yun, Cox, Chawla: "Circadian gene Bmal1 regulates diurnal oscillations of Ly6C(hi) inflammatory monocytes." in: Science (New York, N.Y.), Vol. 341, Issue 6153, pp. 1483-8, (2013) (PubMed).

  • Target
    S100A8 (S100 Calcium Binding Protein A8 (S100A8))
    Alternative Name
    S100A8 (S100A8 Products)
    Synonyms
    60B8AG antibody, CAGA antibody, CFAG antibody, CGLA antibody, CP-10 antibody, L1Ag antibody, MA387 antibody, MIF antibody, MRP8 antibody, NIF antibody, P8 antibody, Mrp8 antibody, S100A8 antibody, 60B8Ag antibody, AI323541 antibody, B8Ag antibody, CFAg antibody, Caga antibody, p8 antibody, MRP-8 antibody, S100 calcium binding protein A8 antibody, S100 calcium binding protein A8 (calgranulin A) antibody, S100A8 antibody, S100a8 antibody
    Background

    Synonyms: P8, MIF, NIF, CAGA, CFAG, CGLA, L1Ag, MRP8, CP-10, MA387, 60B8AG, Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8, MRP-8, S100 calcium-binding protein A8, Urinary stone protein band A, S100A8

    Background: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect, regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK.

    Gene ID
    6279
    UniProt
    P05109
    Pathways
    Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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