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REST antibody (AA 401-450)

REST Reactivity: Human, Rat, Mouse WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN747683
  • Target See all REST Antibodies
    REST (RE1-Silencing Transcription Factor (REST))
    Binding Specificity
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 401-450
    Reactivity
    • 44
    • 9
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Host
    • 43
    • 1
    Rabbit
    Clonality
    • 43
    • 1
    Polyclonal
    Conjugate
    • 23
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This REST antibody is un-conjugated
    Application
    • 30
    • 20
    • 10
    • 7
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    KO-Validated
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Cow,Pig,Horse,Chicken,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human NRSF
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Li, Cheng, Xu, Zhang, Tokat, Dolios, Ramakrishnan, Shen, Wang, Xu: "The transcriptional coactivator Eya1 exerts transcriptional repressive activity by interacting with REST corepressors and REST-binding sequences to maintain nephron progenitor identity." in: Nucleic acids research, Vol. 50, Issue 18, pp. 10343-10359, (2022) (PubMed).

    Zhang, Wang, Lu, Wang, Yuan, Yan, Cai, Chang, Zheng, Wu, Zhou: "REST regulates the cell cycle for cardiac development and regeneration." in: Nature communications, Vol. 8, Issue 1, pp. 1979, (2018) (PubMed).

    Zhang, Wu, Wang, Wang, Lu, Nechiporuk, Floss, Greally, Zheng, Zhou: "Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts." in: Nucleic acids research, Vol. 45, Issue 6, pp. 3102-3115, (2017) (PubMed).

  • Target
    REST (RE1-Silencing Transcription Factor (REST))
    Alternative Name
    NRSF/REST (REST Products)
    Synonyms
    NRSF antibody, XBR antibody, 2610008J04Rik antibody, AA407358 antibody, xbr antibody, nrsf antibody, rest antibody, resta antibody, REST antibody, RE1 silencing transcription factor antibody, RE1-silencing transcription factor antibody, RE1-silencing transcription factor S homeolog antibody, REST antibody, Rest antibody, rest.S antibody, rest antibody, LOC615792 antibody
    Background

    Synonyms: XBR, NRSF, RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor, REST

    Background: Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.

    Gene ID
    5978
    UniProt
    Q13127
    Pathways
    Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity
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