TBK1 antibody (pSer172)

Catalog No. ABIN746363

Product Details anti-TBK1 Antibody

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Binding Specificity: pSer172

Reactivity: Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBK1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This phosphorylation site is homologous across the listed species.

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human TBK1 around the phosphorylation site of Ser172

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TBK1 antibody (ABIN746363)

Hu, Pan, Cheng, Zhang, Wang, Chen, Zhu, Liu, Yang, Wu, Zhao, Huang, Zhang, Mao, Ou, Yu, Zhang, Liu, Tian, Pan, Rehman, Yin, Jia: "Autophagy Promotes Duck Tembusu Virus Replication by Suppressing p62/SQSTM1-Mediated Innate Immune Responses In Vitro." in: Vaccines, Vol. 8, Issue 1, (2020) (PubMed).

Wan, Chen, Li, Pang, Chen, Nie, Pan, Qiao, Bao: "Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction." in: International journal of biological macromolecules, Vol. 138, pp. 198-206, (2020) (PubMed).

Takahashi, Loo, Okada, Kamachi, Watanabe, Wakita, Watanabe, Kawamoto, Miyata, Barber, Ohtani, Hara: "Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells." in: Nature communications, Vol. 9, Issue 1, pp. 1249, (2018) (PubMed).

Target Details for TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Alternative Name: TBK1 (TBK1 Products)

Synonyms: nak antibody, t2k antibody, TBK1 antibody, wu:fk70c05 antibody, zgc:136548 antibody, NAK antibody, T2K antibody, 1200008B05Rik antibody, AI462036 antibody, AW048562 antibody, TANK binding kinase 1 L homeolog antibody, TANK binding kinase 1 antibody, TANK-binding kinase 1 antibody, tbk1.L antibody, TBK1 antibody, tbk1 antibody, Tbk1 antibody

Background:

Synonyms: NAK, T2K, Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, TANK-binding kinase 1, TBK1

Background: Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. Phosphorylates Borna disease virus (BDV) P protein.

Gene ID: 29110

UniProt: Q9UHD2

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome