RPS6KA3 antibody (pSer227)

Catalog No. ABIN745268

Product Details anti-RPS6KA3 Antibody

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Binding Specificity: pSer227

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KA3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody will cross react with Ribosomal protein S6 kinase alpha-1 when phosphorylated at Ser221 and Ribosomal protein S6 kinase alpha-6 at Ser232.

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human RSK2 around the phosphorylation site of Ser227

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RPS6KA3

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Alternative Name: RSK2 (RPS6KA3 Products)

Synonyms: HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, CLS antibody, HU-3 antibody, ISPK-1 antibody, MAPKAPK1B antibody, MRX19 antibody, RSK2 antibody, S6K-alpha3 antibody, p90-RSK2 antibody, pp90RSK2 antibody, MPK-9 antibody, Rsk2 antibody, RGD1563860 antibody, id:ibd1103 antibody, rps6ka3 antibody, si:ch211-114c12.12 antibody, wu:fk86e02 antibody, zgc:56006 antibody, zgc:158386 antibody, ribosomal protein S6 kinase A1 antibody, ribosomal protein S6 kinase A3 antibody, ribosomal protein S6 kinase polypeptide 3 antibody, ribosomal protein S6 kinase a, polypeptide 3a antibody, ribosomal protein S6 kinase, polypeptide 3b antibody, RPS6KA1 antibody, RPS6KA3 antibody, Rps6ka3 antibody, rps6ka3a antibody, rps6ka3b antibody

Background:

Synonyms: Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, 90 kDa ribosomal protein S6 kinase 3, p90-RSK 3, p90RSK3, Insulin-stimulated protein kinase 1, ISPK-1, MAP kinase-activated protein kinase 1b, MAPK-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, Ribosomal S6 kinase 2, RSK-2, pp90RSK2, RPS6KA3, ISPK1, MAPKAPK1B, RSK2

Background: Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4).

Gene ID: 6197

UniProt: P51812

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades